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Primed in situ DNA amplification (PIDA)
1Allergic Diseases Section, NIAID, National Institute of Health, Bethesda, Maryland.
Journal of Clinical Laboratory Analysis
|January 1, 1994
Abstract:
A novel PCR in situ protocol is described which can be implemented in one day. An initial amplification step with a single primer pair is followed by an amplification with an internal oligonucleotide probe with labeled nucleotides (dig-dUTP). Detection is then accomplished with an antidigoxigenin antibody and substrate solution. This rapid and sensitive method may be useful in diagnosing low copy DNA in tissue specimens and cells.