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Transforming growth factor-alpha: identification in bovine corpus luteum by immunohistochemistry and northern blot

D K Lobb1, J H Dorrington

  • 1Department of Obstetrics and Gynaecology, McMaster University, Hamilton, Ontario, Canada.

Reproduction, Fertility, and Development
|January 1, 1993
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.