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Related Experiment Videos

[Bovine 17-beta-estradiol during estrous cycle]

F Ectors, J F Beckers, P Ballman

    Comptes Rendus Hebdomadaires Des Seances De L'Academie Des Sciences. Serie D: Sciences Naturelles
    |October 27, 1975
    PubMed
    Summary

    A new radioimmunoassay (RIA) method accurately measures plasma estradiol-17 beta without chromatography. Estradiol-17 beta levels peak at estrus onset, then decline, with minor peaks observed during the cycle.

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    Pregnancy-associated glycoproteins (PAGs) concentrations in water buffaloes (Bubalus bubalis) during gestation and the postpartum period.

    Theriogenology·2017

    Area of Science:

    • Reproductive Endocrinology
    • Hormone Assays

    Context:

    • Accurate measurement of estradiol-17 beta is crucial for understanding reproductive cycles.
    • Traditional methods often require complex chromatographic separation, limiting efficiency.

    Purpose:

    • To develop and validate a direct radioimmunoassay (RIA) for plasma estradiol-17 beta determination.
    • To establish a simplified, highly specific assay for estradiol-17 beta without chromatography.

    Summary:

    • A novel RIA method utilizing a specific antiserum was established for direct plasma estradiol-17 beta measurement.
    • Estradiol-17 beta levels exhibited a significant peak (8.6 pg/ml) at estrus onset, followed by a sharp decrease.
    • Secondary peaks were observed on days 4-5, 12, and occasionally day 8, with a basal level of 1.75 pg/ml.

    Impact:

    • Provides a more efficient and accessible method for estradiol-17 beta quantification.
    • Facilitates detailed studies of hormonal fluctuations during the estrus cycle.
    • Potential for improved diagnostic and research applications in reproductive biology.

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