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Glucose transporter 2 (GLUT 2): expression in specific brain nuclei
C Leloup1, M Arluison, N Lepetit
1Laboratoire de Physiopathologie de la Nutrition, CNRS URA 307, Université Paris VII, France.
Brain Research
|February 28, 1994
Summary
Brain glucose sensors may involve glucose transporter 2 (GLUT 2). This study found GLUT 2 in key brain areas, suggesting its role in regulating feeding behavior and glucose sensing.
Area of Science:
- Neuroscience
- Molecular Biology
- Endocrinology
Background:
- Neurons in the brain detect glucose concentration changes.
- Glucose transporters are potential candidates for these 'glucose sensors'.
Purpose of the Study:
- To investigate if glucose transporter 2 (GLUT 2) functions as a cerebral glucose sensor.
- To determine the localization and distribution of GLUT 2 in the brain.
Main Methods:
- Polymerase chain reaction (PCR) to detect GLUT 2 mRNA.
- Immunocytochemistry and electron microscopy to visualize GLUT 2 protein distribution and cellular localization.
Main Results:
- GLUT 2 mRNA was detected in specific brain nuclei, including those involved in feeding behavior.
- GLUT 2 immunoreactivity was widespread in the brain, localized to astrocytes.
- GLUT 2 was found in regions associated with feeding regulation.
Conclusions:
- Glucose transporter 2 (GLUT 2) is present in the brain and localized to astrocytes.
- The distribution of GLUT 2 in feeding-related brain regions supports its role in cerebral glucose sensing.