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Expression and functional characterisation of a synthetic version of the human D4 dopamine receptor in a stable human
M McHale1, M C Coldwell, N Herrity
1Department of Biotechnology, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
Abstract:
A synthetic version of the human D4 (hD4) dopamine receptor was prepared. The G/C content of the natural gene was reduced by 14% without altering the amino acid composition of the corresponding protein sequence. HEK293 cells were transfected with the synthetic hD4 gene and stable clones resistant to G418 selected. The hD4 receptor expressed from the synthetic gene had identical pharmacological characteristics to the native hD4 receptor [(1991) Nature 350, 610-619; (1992) Nature 358, 149-152]. Functional studies with cells expressing the synthetic hD4 gene indicated negative coupling of this receptor to adenylate cyclase.