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Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure
Published on: August 25, 2017
Guinea pig lung inflammatory cell changes following acute ozone exposure
1Department of Environmental Health Sciences, Johns Hopkins University, Baltimore, Maryland 21205.
Lung
|January 1, 1994
Summary
Ozone (O3) exposure causes lung injury and inflammation. Bronchoalveolar lavage (BAL) alone underestimates early inflammatory cell changes in the lungs compared to tissue digestion methods.
Area of Science:
- Environmental Toxicology
- Pulmonary Medicine
- Cellular Biology
Background:
- Ozone (O3) is a major air pollutant with known pulmonary toxicity.
- Understanding the kinetics of inflammatory cell infiltration is crucial for assessing lung injury.
- Bronchoalveolar lavage (BAL) is a common method, but its ability to capture early tissue-level changes is debated.
Purpose of the Study:
- To establish the time course of inflammatory cell infiltration in guinea pig lungs after ozone exposure.
- To compare inflammatory cell populations recovered by bronchoalveolar lavage (BAL) and collagenase tissue digestion.
- To assess the utility of BAL in reflecting early inflammatory cell dynamics in oxidant-induced lung injury.
Main Methods:
- Guinea pigs were exposed to 2 ppm ozone (O3) for 4 hours.
- Inflammatory cells were quantified using bronchoalveolar lavage (BAL) and collagenase digestion of lung tissue.
- Albumin levels in BAL fluid were measured as an indicator of lung permeability.
- Cell populations (macrophages, neutrophils, eosinophils) were identified and enumerated.
Main Results:
- Ozone exposure increased lung permeability and inflammatory cell infiltration.
- BAL underestimated early neutrophil accumulation in lung tissue compared to collagenase digestion.
- Macrophages and monocytes showed delayed increases in tissue digests, while BAL showed transient increases.
- Lavage-recovered eosinophils increased transiently, but tissue digestion did not detect significant changes.
Conclusions:
- Bronchoalveolar lavage (BAL) alone does not fully represent early inflammatory cell changes in ozone-exposed lungs.
- Collagenase tissue digestion provides a more comprehensive assessment of inflammatory cell infiltration kinetics.
- Combined BAL and tissue digestion methods offer a better understanding of reversible lung injury models.
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