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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Antibody-antigen binding constants determined in solution-phase with the threshold membrane-capture system: binding

K Dill1, M Lin, C Poteras

  • 1Molecular Devices, Menlo Park, California 94025.

Analytical Biochemistry
|February 15, 1994
PubMed

Affinities of various monoclonal and polyclonal antibodies for fluorescein-containing antigens, saxitoxin and ricin, were determined by using a light addressable potentiometric sensor-based system (Threshold). The dissociation constants, determined from Scatchard plots, ranged from 2 x 10(-7) to approximately 3 x 10(-12) M. Dissociation constants for fluorescein and saxitoxin were compared with values determined by independent means. This technique was found to be quick, simple, reproducible, and accurate.

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