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Chiral separation by capillary affinity zone electrophoresis using an albumin-containing support electrolyte
1Analytical Research Center, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan.
Analytical Biochemistry
|February 15, 1994
Summary
Capillary electrophoresis effectively separated pharmaceutical compounds using bovine serum albumin (BSA) as a chiral selector. Optimal conditions involved phosphate buffer at pH 7-8, with BSA concentration and voltage influencing stereoselectivity.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
- Biophysical Chemistry
Background:
- Chiral separations are crucial for pharmaceuticals.
- Capillary electrophoresis offers high-resolution separation.
- Bovine serum albumin (BSA) is a potential chiral selector.
Purpose of the Study:
- To develop capillary electrophoresis methods for chiral separation of pharmaceutical compounds.
- To investigate bovine serum albumin (BSA) as a chiral selector.
- To optimize separation conditions for enantiomers.
Main Methods:
- Capillary affinity zone electrophoresis was employed.
- Bovine serum albumin (BSA) was used as the chiral selector in the electrolyte.
- Phosphate and borate buffers were compared as support electrolytes.
- Effects of BSA concentration, pH, voltage, and chiral additives were studied.
Main Results:
- Phosphate buffer was superior to borate buffer for separating new quinolone bactericidal reagents.
- Effective chiral separations were achieved in the pH range of 7-8.
- Migration and stereoselectivity were modulated by BSA concentration (>0.2% w/v), voltage, and amino acid additives.
Conclusions:
- Capillary electrophoresis with BSA is a viable method for chiral pharmaceutical separations.
- The developed method is adaptable for other chiral compounds and protein binding studies.
- Optimization of BSA concentration, pH, and voltage is key for stereoselective separation.