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Novel high-performance liquid chromatographic method using solid-phase on-line elution for determination of
D Farthing1, D A Sica, I Fakhry
1Division of Clinical Pharmacology, Medical College of Virginia, Richmond 23298-0160.
Journal of Chromatography. B, Biomedical Applications
|March 4, 1994
Abstract:
A novel solid-phase on-line elution HPLC method employing fluorescence detection to measure metolazone in plasma and whole blood has been developed. The method is sensitive and selective for metolazone and linear over a dynamic range of 1-50 ng/ml with a sample requirement of 250 microliters. The limit of quantitation for the method is 1 ng/ml and combined intra- and inter-day accuracy and precision had an error and coefficient of variation of 2.9 and 5.5%, respectively.