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Cytokine release by human bone marrow cells: analysis at the single cell level
D Rohde1, C Wickenhauser, S Denecke
1Institute of Pathology, University of Cologne, Germany.
Virchows Archiv : an International Journal of Pathology
|January 1, 1994
Summary
Erythroid cells, not just immune cells, secrete key regulatory cytokines in bone marrow. This study used the reverse hemolytic plaque assay to identify these cytokine-producing cells, revealing erythropoietic cells as major contributors to hematopoietic regulation.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Bone marrow microenvironment regulation involves growth factors.
- Identifying cytokine-secreting cells is challenging due to cell heterogeneity.
Purpose of the Study:
- To analyze cytokine release from normal human bone marrow cells at the single-cell level.
- To identify the specific cell types responsible for secreting key hematopoietic growth factors.
Main Methods:
- Employed the reverse hemolytic plaque assay (RHPA) to detect cytokine secretion.
- Combined RHPA with immunocytochemical and enzymatic staining to identify cell types.
- Examined release of interleukin-1 alpha (IL-1α), IL-3, IL-6, and granulocyte-monocyte colony-stimulating factor (GM-CSF).
Main Results:
- Erythropoietic cells (Glycophorin C-positive) were the largest fraction (up to 89%) of cytokine-secreting cells.
- Neutrophil granulocytes and monocytes/macrophages also secreted cytokines, but in lower proportions.
- Cytokine secretion increased significantly after stimulation with recombinant human-IL-1α and rhGM-CSF.
Conclusions:
- Erythropoietic cells play a significant role in producing cytokines that regulate the bone marrow microenvironment.
- The RHPA is a valuable tool for measuring cytokine release from hematopoietic cells and other tissues.
- This finding challenges traditional views by highlighting erythropoietic cells as key cytokine producers in hematopoiesis.