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Overexpression of the Raf-1 proto-oncogene in human myeloid leukemia
C A Schmidt1, H Oettle, W D Ludwig
1Abteilung für Innere Medizin und Poliklinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, F.R.G.
Leukemia Research
|June 1, 1994
Abstract:
The Raf-1 protein, a cytoplasmic serine/threonine kinase, plays an important role in signal transduction pathways. In order to examine the role of Raf-1 in human myeloid leukemia, we determined raf-1 mRNA expression by Northern blot analysis in blast cell samples from 27 acute myeloid leukemia (AML) cases and peripheral blood mononuclear cells from six healthy donors. A normal raf-1 transcript size was detected in all cases investigated. However, overexpression of raf-1 mRNA was found in 2 of 27 AML cases, both of which were erythroleukemias (AML, FAB M6).