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MBR1 and MBR3, two related yeast genes that can suppress the growth defect of hap2, hap3 and hap4 mutants

B Daignan-Fornier1, C C Nguyen, P Reisdorf

  • 1Laboratoire de Génétique Moléculaire, Université Paris-Sud, Orsay, France.

Molecular & General Genetics : MGG
|June 3, 1994
PubMed

Insights

Two novel yeast genes, MBR1 and MBR3, were identified as multicopy suppressors for growth defects. Their products likely share a common function in yeast, with MBR1 playing a more significant role.

Area of Science:

  • Molecular biology
  • Yeast genetics

Background:

  • The HAP2/3/4 complex is crucial for transcriptional activation in yeast.
  • Understanding gene regulation and compensatory mechanisms is vital in molecular biology.

Purpose of the Study:

  • To identify novel genes that can suppress growth defects associated with the HAP2 transcriptional activator.
  • To characterize the function and relationship of the newly discovered genes, MBR1 and MBR3.

Main Methods:

  • Isolation of multicopy suppressors for yeast growth defects.
  • Gene overexpression and null mutant analysis.
  • Nucleotide sequencing and mutagenesis of MBR1 and MBR3.
  • Phenotypic analysis on glycerol medium.

Main Results:

  • MBR1 and MBR3 were identified as multicopy suppressors of HAP2, hap3, and hap4 null mutant growth defects.
  • MBR1 and MBR3 encode homologous, serine-rich proteins.
  • MBR1 disruption caused a partial growth defect on glycerol, while MBR3 disruption had no major effect.
  • A synthetic phenotype in the double disruptant suggested a shared function between MBR1 and MBR3.

Conclusions:

  • MBR1 and MBR3 encode proteins involved in a common cellular function in yeast.
  • These genes represent novel components potentially interacting with or regulating pathways involving the HAP2/3/4 complex.

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