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Evidence that the epidermal growth factor receptor on host cells confers reovirus infection efficiency

J E Strong1, D Tang, P W Lee

  • 1Department of Microbiology & Infectious Diseases, University of Calgary Health Sciences Centre, Alberta, Canada.

Virology
|November 1, 1993
PubMed

Insights

Epidermal growth factor (EGF) receptors enhance host cell susceptibility to reovirus infection. Functional EGF receptors are required, suggesting a link between reovirus entry and EGF receptor signaling pathways.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Reovirus infection relies on host cell surface molecules.
  • Epidermal growth factor (EGF) receptors role in reovirus susceptibility is unclear.

Purpose of the Study:

  • To investigate the role of EGF receptors in host cell susceptibility to reovirus.

Main Methods:

  • Utilized mouse cell lines NR6 and B82, with and without EGF receptor expression.
  • Assessed reovirus infection efficiency via cytopathic effects, viral protein synthesis, and plaque titration.
  • Compared infection in cells expressing functional vs. kinase-inactive EGF receptors.

Main Results:

  • Cells expressing EGF receptors showed significantly higher reovirus susceptibility.
  • Infection efficiency enhancement was dependent on a functional EGF receptor kinase domain.
  • No differences in virus binding or internalization were observed between cell lines.

Conclusions:

  • EGF receptor expression increases host cell susceptibility to reovirus.
  • Functional EGF receptors are critical for enhanced reovirus infection.
  • Reovirus infection is closely linked to EGF receptor-mediated signal transduction.

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