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Effect of loratadine on human eosinophil function in vitro
1Allergic Disease Center, Creighton University School of Medicine, Omaha, Nebraska.
Annals of Allergy
|October 1, 1993
Summary
Loratadine, a non-sedating antihistamine, directly inhibits key eosinophil functions like chemotaxis and superoxide generation in vitro. This suggests loratadine
Area of Science:
- Immunology
- Pharmacology
- Allergy Research
Background:
- Eosinophils play a crucial role in allergic inflammation.
- Histamine H1 antagonists are widely used for allergy treatment.
- Understanding the direct effects of antihistamines on immune cells is important.
Purpose of the Study:
- To investigate the in vitro effects of loratadine on eosinophil functions.
- To determine if loratadine impacts eosinophil chemotaxis, superoxide anion generation, and ECP release.
Main Methods:
- Purified eosinophils from allergic patients were used.
- In vitro assays measured chemotaxis, superoxide anion (O2-) generation, and eosinophil cationic protein (ECP) release.
- Platelet-activating factor (PAF) was used to induce eosinophil activation.
Main Results:
- Loratadine significantly reduced PAF-induced eosinophil chemotaxis at therapeutic concentrations.
- Loratadine also attenuated PAF-induced superoxide anion (O2-) generation.
- Loratadine did not affect PAF-induced eosinophil cationic protein (ECP) release.
Conclusions:
- Loratadine exhibits a direct inhibitory effect on eosinophil activation.
- These findings support the anti-allergic properties of loratadine.
- Loratadine may be beneficial in managing allergic disorders by modulating eosinophil function.