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In vitro postantibiotic effect following repeated exposure to imipenem, temafloxacin, and tobramycin

B J McGrath1, C R Marchbanks, D Gilbert

  • 1Antiinfective Pharmacology Research Unit, University of Rhode Island College of Pharmacy, Roger Williams Medical Center, Providence 02908.

Antimicrobial Agents and Chemotherapy
|August 1, 1993
PubMed

Insights

Repeated antibiotic exposure alters the postantibiotic effect (PAE) in Pseudomonas aeruginosa. While imipenem maintained its PAE, temafloxacin

Area of Science:

  • Microbiology
  • Pharmacology
  • Bacterial resistance

Background:

  • The postantibiotic effect (PAE) is crucial for understanding antibiotic efficacy.
  • Pseudomonas aeruginosa is an opportunistic pathogen with clinical significance.
  • Variations in PAE with repeated drug exposure are not fully understood.

Purpose of the Study:

  • To investigate the impact of multiple antibiotic exposures on the PAE in Pseudomonas aeruginosa.
  • To compare the PAE of imipenem, temafloxacin, and tobramycin after repeated dosing.

Main Methods:

  • Determined the PAE in Pseudomonas aeruginosa following three consecutive 2-hour exposures to imipenem, temafloxacin, and tobramycin.
  • Monitored bacterial regrowth after each drug exposure cycle.

Main Results:

  • Imipenem consistently exhibited a PAE and bactericidal effect after each exposure cycle.
  • Temafloxacin showed an increasing PAE with repeated exposure (1.8 to > 5 hours).
  • Tobramycin's PAE diminished with repeated exposure, disappearing by the third cycle (0.9 to 0 hours).

Conclusions:

  • The in vitro postantibiotic effect can change within a bacterial strain following multiple cycles of drug exposure and regrowth.
  • Antibiotic-specific responses highlight the dynamic nature of PAE in Pseudomonas aeruginosa.
  • These findings have implications for optimizing antibiotic treatment strategies.

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