Determination of antidromic excitation by the collision test: problems of interpretation

Extracellular unit recordings were made from pontine reticular neurons in the cat and cells of the motor cortex in monkeys. In all cases, the characteristics of responses to electrical stimulation were studied using the tests of invariance of latency, high frequency following, and collision for determining the orthodromic or antidromic nature of the responses. The results of these tests show that their conclusions are not always consistent. A systematic error was found between the experimental and predicted values of the collision interval. It is argued that this error is due to differences in the application of measured parameters in calculating the collision interval. The collision test can be considerably improved by repeating the test with stimuli of progressively greater strengths.