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Developmental and circadian pattern of rubisco activase mRNA accumulation in apple plants
B Watillon1, R Kettmann, P Boxus
1Unité de Biologie Moléculaire et Physiologie Animale, Faculté des Sciences Agronomiques de Gembloux, Belgium.
Abstract:
An apple cDNA encoding the precursor of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) activase has been characterized. Using this cDNA as a probe, leaf-specific and light-regulated accumulation of corresponding transcripts was detected. Rubisco activase transcripts also turned out to accumulate at growing levels during apple leaf development, to reach a maximum in fully expanded leaves. In contrast, chlorophyll a/b-binding protein (Cab) and rubisco large subunit mRNA levels reach a maximum earlier in the course of leaf development. Moreover, the accumulation of rubisco activase messengers appeared to follow an oscillating circadian rhythm qualitatively similar to that observed for Cab mRNA levels.