[A modification of the ortho-toluidine method for determining glucose in the blood]

Modification of the orthotoluidine technique is associated with the necessity to examine blood glucose levels in mice, its dynamics analysis (by 5-6 points), which greatly expands the possibility of an experiment on screening new hypoglycemic agents. Addition of tert-butanol (isobutamol) facilitates the interaction of orthotoluidine with glucose, by enhancing the sensitivity and accuracy of the technique and allowing blood microquantities (10-20 microliters per determination) to be analysed. In terms of its accuracy and reliability, the proposed modification is not virtually inferior to expensive enzymatic techniques. The findings suggest that the modified orthotoluidine technique may be useful in the analysis of glucose levels in dynamics in small laboratory animals.