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Analysis and sorting of chromosomes by flow cytometry: new trends
P Métézeau1, A Schmitz, G Frelat
1Unité de Biochimie Cellulaire, INSERM-Pasteur et CNRS URA 1129, Institut Pasteur, Paris.
Biology of the Cell
|January 1, 1993
Abstract:
Flow cytogenetic is widely used since 1975, and essentially contributes to karyotype analysis and chromosome sorting. The principles of experimentation and its possibilities and limitations are now well known. Recently several new technologies have appeared. What attitude should the cytometrist adopt regarding PCR, microdissection of chromosomes, in situ hybridization, slit-scan flow cytometry or image analysis?