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The effect of protein kinase C modulators on endogenous protein phosphorylation in primate kidney
N Laminski1, K Hammond, A Meyers
1Department of Medical Biochemistry, University of the Witwatersrand, Parktown, Johannesburg, South Africa.
Biochemistry and Molecular Biology International
|August 1, 1993
Abstract:
The effects of modulators of protein kinase C on protein phosphorylation were studied in primate kidney. Phosphorylation of specific proteins, investigated using electrophoresis and autoradiography, indicated that in the absence of modulators eight major proteins were phosphorylated in membrane of baboon kidney; modulators enhanced a possible endogenous substrate of 41.8 +/- 2.3 kDa. In cytosol, eight major proteins were phosphorylated in the absence of modulators; a band of mass 110.4 +/- 13.8 kDa was enhanced with modulators added. Similar results were found in human kidney cytosol.