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Updated: Aug 18, 2026

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
Published on: November 23, 2019
Surface charge modifications do not affect the hydrolytic activity of membrane-bound pyrophosphatase of
1Departamento de Bioenergética, Universidad Nacional Autónoma de México, México, D.F.
Abstract:
The surface charge of the membrane of chromatophores of Rhodospirillum rubrum was modified by two methods: fusion of liposomes with the membrane of the chromatophore by changing the pH and by incubating chromatophores in the presence of cationic or anionic detergents. The hydrolytic activity of membrane-bound pyrophosphatase, on surface charge modified chromatophores, did not change the Km of the enzyme for its substrate (Mg-PPi2-) nor the activation effect of free Mg2+ on the hydrolytic activity. This membrane enzyme is not regulated by surface charge.
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