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Published on: October 28, 2012
Inhibitory effects of hyaluronan on neutrophil-mediated cartilage degradation
K Tobetto1, K Nakai, M Akatsuka
1Research Laboratories, Maruho Co., Ltd., Osaka, Japan.
Abstract:
The effects of hyaluronan on neutrophil-mediated cartilage degradation were studied in an in vitro model. Rat peritoneal neutrophils were incubated for up to 18 h with the neutralized cartilage of bovine nasal septa using N-formyl-L-methionyl-L-leucyl-L-phenylalanine combined with cytochalasin B, or opsonized zymosan, as a stimulation agent of neutrophils. Hyaluronan inhibited the neutrophil-mediated cartilage degradation by reducing the release of sulfated glycosaminoglycans from the cartilage. Inhibitory effects were dependent on concentration and molecular weight of hyaluronan. These results suggest that hyaluronan with a high molecular weight plays an important role in protecting the articular cartilage in inflamed joints from neutrophil injury.
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