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3' non-coding region sequences in eukaryotic messenger RNA

N J Proudfoot, G G Brownlee

|September 16, 1976

Summary

A conserved sequence (A-A-U-A-A-A) was found near the poly (A) tail in multiple messenger RNA molecules across species. This suggests significant evolutionary conservation of this important RNA region.

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Area of Science:

Molecular Biology
Genetics
Evolutionary Biology

Background:

Messenger RNA (mRNA) molecules play a crucial role in protein synthesis.
The 3' non-coding region of mRNA, including the poly (A) tail, influences mRNA stability and translation.
Understanding conserved sequences in mRNA can provide insights into gene regulation and evolution.

Purpose of the Study:

To identify conserved sequences within the 3' non-coding regions of specific eukaryotic mRNAs.
To investigate the evolutionary significance of these conserved sequences.
To examine the proximity of conserved sequences to the 3'-terminal poly (A) sequence.

Main Methods:

Purification of specific messenger RNA molecules (alpha- and beta-globulin mRNAs, immunoglobulin light chain mRNA, ovalbumin mRNA) from different species (rabbit, human, mouse, chicken).
Sequence analysis of the 3' non-coding regions of these purified mRNAs.
Homology comparison of the 3' non-coding regions to assess evolutionary conservation.

Main Results:

The sequence A-A-U-A-A-A was identified in six different purified messenger RNA molecules.
This conserved sequence is located approximately 20 residues upstream of the 3'-terminal poly (A) sequence.
Significant homology (85%) was found in the 3' non-coding regions of rabbit and human globulin mRNAs.

Conclusions:

The A-A-U-A-A-A sequence represents a conserved element in the 3' non-coding region of various eukaryotic mRNAs.
The high homology in the 3' non-coding regions of globulin mRNAs suggests strong evolutionary pressure to maintain this sequence.
This conserved region likely plays a functional role in mRNA processing, stability, or translation, warranting further investigation.

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