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Transcriptional interference and gene orientation in yeast: noncoding RNA connections
1Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Cold Spring Harbor Symposia on Quantitative Biology
|April 7, 2011
Summary
Gene transcription requires precise termination to prevent interference. Noncoding RNAs add complexity, impacting gene regulation and silencing in yeast and potentially higher eukaryotes.
Area of Science:
- Molecular Biology
- Genetics
- Gene Regulation
Background:
- Yeast's compact genome necessitates defined transcription units to prevent read-through transcription.
- Failure in transcription termination can lead to transcriptional interference, affecting downstream genes.
- Noncoding RNAs significantly increase the complexity of gene regulation via transcriptional interference.
Purpose of the Study:
- To explore the mechanisms and implications of transcriptional interference in gene regulation.
- To investigate the role of noncoding RNAs in transcriptional interference and gene silencing.
- To compare transcriptional interference strategies in yeast with those in higher eukaryotes.
Main Methods:
- Analysis of budding and fission yeast gene systems.
- Investigating the impact of noncoding RNA synthesis on gene regulation.
- Comparative study of gene regulation mechanisms across different eukaryotic organisms.
Main Results:
- Transcriptional interference is a key regulatory mechanism in yeast, influenced by noncoding RNAs.
- Mechanisms include blocking RNA polymerase access or forming repressive chromatin.
- Fission yeast utilizes read-through transcription to trigger RNA interference and gene silencing.
- Similar strategies involving noncoding RNAs and transcriptional interference are suggested in mammals.
Conclusions:
- Transcriptional interference is a conserved gene regulatory strategy across eukaryotes.
- Noncoding RNAs play a crucial role in modulating gene expression through interference and silencing.
- Understanding yeast systems provides insights into gene regulation in complex genomes.
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