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Related Experiment Videos

The cDNA sequence encoding human protein kinase C-zeta

J L Barbee1, S L Deutscher, C R Loomis

  • 1Sphinx Pharmaceuticals, Durham, NC 27707

Gene
|October 15, 1993
PubMed
Summary

Researchers isolated a human protein kinase C-zeta (PKC-zeta) complementary DNA (cDNA). This finding provides insights into human PKC-zeta, showing high similarity to rodent PKC-zeta sequences.

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Area of Science:

  • Molecular Biology
  • Neuroscience

Background:

  • Protein kinase C-zeta (PKC-zeta) is involved in various cellular signaling pathways.
  • Understanding the human PKC-zeta sequence is crucial for comparative genomics and studying its role in neurological functions.

Purpose of the Study:

  • To isolate and characterize the complementary DNA (cDNA) encoding human protein kinase C-zeta (PKC-zeta).
  • To compare the human PKC-zeta sequence with its rodent counterparts.

Main Methods:

  • Screening of a human frontal cortex complementary DNA (cDNA) library using plaque-screening and PCR methods.
  • Deduction of the amino acid sequence from the isolated cDNA clone.

Main Results:

  • A 1779-bp complementary DNA (cDNA) encoding human protein kinase C-zeta (PKC-zeta) was successfully isolated.
  • The deduced 592-amino-acid sequence of human PKC-zeta exhibited 95-96% identity to rat and mouse PKC-zeta sequences.

Conclusions:

  • The isolation of human PKC-zeta cDNA provides a valuable resource for further research.
  • High sequence homology suggests conserved function of PKC-zeta across mammalian species, particularly in the brain.

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