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Affinity chromatography with nucleic acid polymers

H W Jarrett1

  • 1Department of Biochemistry, University of Tennessee, Memphis 38168.

Journal of Chromatography
|August 25, 1993
PubMed
Summary
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This review covers polynucleotide-based affinity chromatography, a key technique for isolating nucleic acids and associated proteins. It highlights applications in the biomedical sciences, including mRNA purification and DNA-binding protein characterization.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chromatography

Background:

  • Affinity chromatography uses immobilized molecules to purify targets.
  • Polynucleotides serve as effective ligands for purifying nucleic acids and proteins.
  • This technique is crucial in molecular biology and biomedical research.

Purpose of the Study:

  • To review column chromatography using immobilized polynucleotides.
  • To discuss applications in isolating polynucleotides and binding proteins.
  • To explore the use of DNA affinity chromatography in various biological processes.

Main Methods:

  • Review of literature on polynucleotide-immobilized chromatography.
  • Discussion of applications in isolating poly(A) mRNA using oligo(dT) or oligo(dU) supports.

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  • Examination of DNA affinity chromatography for purifying DNA-binding proteins.

    • Main Results:

    • Polynucleotide affinity chromatography is effective for isolating nucleic acids and proteins.
    • Specific applications include mRNA purification and characterization of DNA-binding proteins.
    • Development of DNA supports for high-performance liquid chromatography is noted.

    Conclusions:

    • Polynucleotide affinity chromatography is a valuable tool in the biomedical sciences.
    • Current applications are widespread, with potential for future advancements.
    • DNA affinity chromatography shows promise for further research and applications.