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A rapid and simple method for counting crevicular polymorphonuclear leucocytes

E Andersen1, G Cimasoni

  • 1Division of Oral Physiopathology and Periodontology, Dental School, Faculty of Medicine, University of Geneva, Switzerland.

Journal of Clinical Periodontology
|October 1, 1993
PubMed
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A new, simple method using Durapore strips effectively harvests and quantifies crevicular polymorphonuclear leucocytes (CPMNs). This technique offers a more accessible approach for periodontal research and clinical assessment of inflammation.

Area of Science:

  • Periodontology
  • Cell Biology
  • Biomaterials

Background:

  • Current methods for counting crevicular polymorphonuclear leucocytes (CPMNs) are often complex, require specialized equipment, and yield only semi-quantitative results.
  • Accurate enumeration of CPMNs is crucial for assessing periodontal inflammation and disease activity.

Purpose of the Study:

  • To develop and validate a novel, simple, and quantitative method for counting crevicular polymorphonuclear leucocytes (CPMNs) using Durapore strips.
  • To establish a more accessible technique for assessing periodontal inflammation.

Main Methods:

  • Durapore strips were utilized to harvest cells from periodontal crevices and pockets.
  • Harvested cells were released from the strips by shaking in a buffer solution (PBS).
  • Liberated polymorphonuclear leucocytes (PMNs) were counted using a Neubauer chamber.

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Main Results:

  • Over 90% of harvested cells were released from Durapore strips.
  • Sequential samplings demonstrated decreasing CPMN counts.
  • CPMN counts significantly decreased after periodontal therapy.
  • Bleeding sites on probing exhibited significantly higher CPMN counts than non-bleeding sites.
  • Deeper periodontal pockets contained significantly more CPMNs.

Conclusions:

  • The Durapore strip method provides a valid, simple, and quantitative approach for enumerating crevicular polymorphonuclear leucocytes (CPMNs).
  • This technique offers a practical alternative for clinical and research settings to assess periodontal inflammation.