Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Thyroxine and triiodothyronine]

A Pinchera, N Ambrosino, C Mammoli

    Annali Dell'Istituto Superiore Di Sanita
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    This study evaluates methods for measuring thyroid hormones, thyroxine (T4) and triiodothyronine (T3). Radioimmunoassay (RIA) is highlighted as advantageous for T3 assessment, with established normal ranges for various patient groups.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The size of the community rather than its geographical location better defines the risk of iodine deficiency: results of an extensive survey in Southern Italy.

    Journal of endocrinological investigation·2013
    Same author

    Frequency of the GPR7 Tyr135Phe allelic variant in lean and obese subjects.

    Journal of endocrinological investigation·2013
    Same author

    The effect of voluntary iodine prophylaxis in a small rural community: the Pescopagano survey 15 years later.

    The Journal of clinical endocrinology and metabolism·2013
    Same author

    Inverse relationship of food and alcohol intake to sleep measures in obesity.

    Nutrition & diabetes·2013
    Same author

    In vivo assessment about the effects of a diet containing iodine-enriched foodstuffs. A pilot study in 30 volunteers.

    Journal of endocrinological investigation·2012
    Same author

    Foreword.

    Journal of endocrinological investigation·2012
    Same journal

    External quality assessment programs in cytogenomics in the Lombardy Region, Italy (2018-2024): performance and trends.

    Annali dell'Istituto superiore di sanita·2026
    Same journal

    Spontaneous orienting of untrained companion dogs naïve to human epilepsy toward odor samples from an unfamiliar human in a controlled non-social paradigm: a proof-of-concept.

    Annali dell'Istituto superiore di sanita·2026
    Same journal

    Addressing food and nutrition insecurity in European countries: a scoping review of strategies and policies.

    Annali dell'Istituto superiore di sanita·2026
    Same journal

    Women's experiences of membrane sweeping for labour induction: a phenomenological qualitative study.

    Annali dell'Istituto superiore di sanita·2026
    Same journal

    Evaluating the usefulness of the Mediterranean Diet Quality Index (KIDMED) in assessing eating habits and nutritional status among preschool children.

    Annali dell'Istituto superiore di sanita·2026
    Same journal

    Optimizing sample preparation for homogeneous mycotoxin distribution in official control analyses.

    Annali dell'Istituto superiore di sanita·2026
    See all related articles

    Area of Science:

    • Endocrinology and Metabolism
    • Clinical Chemistry
    • Hormone Assays

    Context:

    • Established clinical value of serum thyroxine (T4) and triiodothyronine (T3) measurements for thyroid function assessment.
    • Examination of current methods for determining circulating thyroid hormones.
    • Significance of the free thyroxine index in evaluating thyroid function, especially in conditions affecting thyroxine-binding protein capacity.

    Purpose:

    • To examine the properties of available methods for assessing circulating thyroid hormones (T4 and T3).
    • To report experience with a radioimmunoassay (RIA) method for total T3 determination.
    • To establish normal serum T3 ranges in various physiological and pathological conditions.

    Summary:

    • Radioimmunoassay (RIA) and displacement analysis for serum T4 yield similar results.

    Related Experiment Videos

  • Elevated or subnormal total T4 levels can occur in euthyroid states due to altered thyroxine-binding protein capacity, where the free thyroxine index remains normal.
  • A simple RIA method for total T3 is presented, with mean serum T3 values reported for normal, hyperthyroid, hypothyroid subjects, pregnant women, and those on contraceptives. Normal ranges for T3 show variability across laboratories.

    • Impact:

    • Highlights the importance of the free thyroxine index for accurate thyroid function evaluation.
    • Demonstrates the utility of RIA for serum T3 measurement, providing reference values.
    • Underscores the need for each laboratory to establish its own normal range for circulating T3 due to inter-laboratory variability.