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Cytotoxicity of retrofill materials
G R Bruce1, N J McDonald, R J Sydiskis
1Department of Endodontics, Baltimore College of Dental Surgery, Dental School, University of Maryland at Baltimore 21201.
Journal of Endodontics
|June 1, 1993
Summary
Dentin bonding agents and endodontic retrofilling materials were evaluated for cytotoxicity. Most materials were cytotoxic, with primers and cleansers showing significant inhibition zones, while amalgam showed increasing cytotoxicity over time.
Area of Science:
- Biomaterials Science
- Dental Materials
- Cell Biology
Background:
- Dentin bonding agents offer advantages like reduced microleakage and improved marginal adaptation for composite resin restorations.
- Their potential use as endodontic retrofilling materials necessitates evaluation of their cytotoxic effects on vital tissues.
Purpose of the Study:
- To assess the cell cytotoxicity of various dental materials, including amalgam, dentin bonding agents, and endodontic retrofilling materials.
Main Methods:
- VERO cells were cultured and overlaid with a medium-agarose mixture containing a vital stain.
- Dental materials were exposed to the cell monolayers immediately or after aging in a buffered saline solution.
- Cytotoxicity was quantified by measuring zones of cell inhibition around the material samples at various time points.
Main Results:
- Initially, all tested materials, except amalgam and Tenure components, exhibited cytotoxicity.
- Dentin bonding primers and cleansers demonstrated mean zones of inhibition of 13.2 mm and 40.0 mm, respectively.
- Amalgam displayed a progressive increase in cytotoxicity, from 0.0 mm at 24 hours to 12.0 mm at 30 days.
Conclusions:
- Most dentin bonding agents and retrofilling materials evaluated possess cytotoxic potential.
- Amalgam's cytotoxicity increases over prolonged exposure periods.
- Further investigation is required to determine the clinical safety of these materials in endodontic applications.