Related Experiment Videos
Quantitation of cannabinoids in biological specimens using probability based matching GC/MS
NIDA Research Monograph
|May 1, 1976
Summary
Urine analysis detected delta-9-THC and related compounds, with levels exceeding controls. Specific fractions identified delta9-THC-11-oic acid and other hydroxylated THC metabolites.
Area of Science:
- Forensic Toxicology
- Analytical Chemistry
- Pharmacology
Background:
- Cannabinoid analysis in biological samples is crucial for forensic and clinical toxicology.
- Understanding the metabolic profile of cannabinoids aids in interpreting drug test results.
Purpose of the Study:
- To characterize and quantify cannabinoid metabolites in urine samples.
- To differentiate between control and potentially exposed individuals based on metabolite profiles.
Main Methods:
- Urine samples were subjected to extraction using hexane and ether.
- Fractions were analyzed for delta-9-tetrahydrocannabinol (delta9-THC), cannabinol (CBN), and various hydroxylated metabolites.
- Gas chromatography-mass spectrometry (GC-MS) or similar analytical techniques were employed for identification and quantification.
Main Results:
- Hexane fractions showed low delta9-THC (approx. 10 ng/ml) and CBN (approx. 12-25 ng/ml) with K-scores above control urine values.
- Ether-extractable neutral fraction contained 8alpha, 11-dihydroxy-delta9-THC (25-150 ng/ml) and suggested 8beta-hydroxy-delta9-THC.
- Ether-extractable, weak acid fraction (E-II) indicated delta9-THC-11-oic acid (100-200 ng/ml) with high confidence.
Conclusions:
- Urine analysis can detect and quantify various delta-9-THC metabolites.
- The presence and levels of these metabolites, particularly delta9-THC-11-oic acid, can serve as biomarkers for cannabinoid exposure.
- Analytical methods employed provide valuable data for forensic interpretation.