Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro evaluation of a continuous-sampling device for pharmacokinetic parameter estimation

C M Paap1, E C Chocas

  • 1University of Texas at Austin, College of Pharmacy 78712.

Therapeutic Drug Monitoring
|August 1, 1993
PubMed
Summary

An osmotic continuous-withdrawal device accurately estimates area under the curve (AUC) in pharmacokinetic studies. This novel sampling method offers a reliable alternative to conventional techniques, simplifying analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Management of otitis media with effusion in young children.

The Annals of pharmacotherapy·1996
Same author

Multiple-dose pharmacokinetics of pentoxifylline and its metabolites during renal insufficiency.

The Annals of pharmacotherapy·1996
Same author

Prospective evaluation of ten methods for estimating creatinine clearance in children with varying degrees of renal dysfunction.

Journal of clinical pharmacy and therapeutics·1995
Same author

Adequacy of recommended aminophylline loading doses in children.

American journal of hospital pharmacy·1994
Same author

Cefpodoxime proxetil: a new, broad-spectrum, oral cephalosporin.

The Annals of pharmacotherapy·1993
Same author

Acute pulmonary edema after polyethylene glycol intestinal lavage in a child.

The Annals of pharmacotherapy·1993

Area of Science:

  • Pharmacokinetics
  • Analytical Chemistry
  • Drug Development

Background:

  • Accurate estimation of area under the curve (AUC) is crucial for pharmacokinetic analysis.
  • Conventional methods for AUC estimation can be labor-intensive and require multiple samples.
  • Novel sampling techniques are needed to improve efficiency and accuracy in pharmacokinetic modeling.

Purpose of the Study:

  • To compare AUC estimates obtained using an osmotic continuous-withdrawal device with those from a conventional trapezoidal method.
  • To evaluate the reliability and efficiency of continuous sampling in an in vitro pharmacokinetic model.

Main Methods:

  • An in vitro pharmacokinetic model was utilized.
  • Experiments were conducted under two conditions with different half-lives (1 h and 2 h).

Related Experiment Videos

  • AUC estimates were compared between the osmotic continuous-withdrawal device and the trapezoidal method.

    • Main Results:

    • AUC estimates from both methods showed a high correlation (r = 0.948, p < 0.0001).
    • Mean AUC values were not statistically different (p = 0.4), with a mean coefficient of variation of 9.5%.
    • The continuous sampling device operated at a consistent zero-order rate, requiring only one analytical sample.

    Conclusions:

    • Continuous sampling using an osmotic device provides a reasonable and reliable estimation of AUC.
    • This method simplifies pharmacokinetic analysis by requiring only a single analytical sample.
    • The device's consistent sampling rate and high correlation with conventional methods support its utility in drug development.