[Glutathione conjugation with diethylmaleate in various brain structures]

It was shown that GSH concentration and glutathione peroxidase activity in hypothalamus (H) and sensomotor cortex (SC) were twice more than in ME. After single or double injection of diethylmaleate (DEM) two-phase change of the GSH level was observed in all the investigated structures. In the first 1-3 h phase, the pool of free GSH decreases by more than 50% as a result of conjugative effect of DEM. In the second phase which is about 3 days long the level of GSH is gradually restored mainly due to the activation of its biosynthesis. In various brain structures conjugation and reduction processes has their own peculiarities: conjugation processes predominate in H and SC while the reduction processes predominate in ME. These processes were almost two times intensified in all the investigated structures under double injection of DEM.