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Microvessel PO2 measurements by phosphorescence decay method

I P Torres Filho1, M Intaglietta

  • 1Department of Applied Mechanics and Engineering Sciences-Bioengineering, University of California, San Diego, La Jolla 92093-0412.

The American Journal of Physiology
|October 1, 1993
PubMed
Summary
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This study presents a novel noninvasive method for measuring intravascular oxygen levels (PO2) in microscopic vessels using phosphorescence. The technique allows for precise PO2 determination in vivo, aiding microcirculation research.

Area of Science:

  • Biomedical Engineering
  • Physiology
  • Optical Imaging

Background:

  • Accurate measurement of tissue oxygen partial pressure (PO2) is crucial for understanding physiological and pathological processes.
  • Existing methods for in vivo PO2 measurement often lack spatial resolution or are invasive.
  • Microvascular oxygenation plays a critical role in tissue health and disease.

Purpose of the Study:

  • To develop and validate a noninvasive system for in vivo microscopic measurement of intravascular and interstitial PO2.
  • To assess the feasibility of using phosphorescence quenching for PO2 determination in microcirculation.
  • To enable simultaneous measurement of microvessel diameter and red blood cell velocity.

Main Methods:

  • Utilized palladium-porphyrins bound to albumin as a phosphorescent probe, injected intravenously.

Related Experiment Videos

  • Employed epi-illumination with a strobe xenon arc to excite phosphorescence.
  • Measured phosphorescence decay using a photomultiplier in defined tissue areas (15 x 30 microns).
  • Applied the Stern-Volmer equation for PO2 calculation based on oxygen-dependent phosphorescence quenching.
  • Validated the system in vitro using saline and blood, and in vivo using hamster skin fold chamber models.

    • Main Results:

    • The system achieved noninvasive, in vivo measurement of intravascular PO2 at the microscopic level (15-100 micron diameter vessels).
    • The method demonstrated capability for interstitial PO2 measurement under specific conditions.
    • PO2 measurements were accurate within the 0-80 mmHg range.
    • Simultaneous transillumination allowed for concurrent measurement of vessel diameter and red blood cell velocity.

    Conclusions:

    • The developed phosphorescence-based system offers a powerful tool for noninvasive, high-resolution in vivo PO2 mapping in microcirculation.
    • This technology can significantly advance research in tissue oxygenation, hemodynamics, and related pathologies.
    • The ability to measure PO2, vessel diameter, and flow velocity simultaneously in the same microvessels provides comprehensive insights into microvascular function.