Preparation of chromosome-specific paints and complete assignment of chromosomes in the pig flow karyotype
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C F Langford1, H Telenius, N G Miller
1AFRC Institute of Animal Physiology & Genetics Research, Babraham Research Station, Babraham, UK.
Animal Genetics
|August 1, 1993
Abstract:
Sorted chromosomes from each of the 20 clusters of the male porcine bivariate flow karyotype were amplified and biotinylated using DOP-PCR. The chromosomes comprising each cluster were identified by fluorescence in situ hybridization (FISH) of the 20 probes to R-banded male pig metaphase spreads. A standard flow karyotype for the pig is presented.