Related Experiment Videos
Time course changes in cytoskeletal structures of cultured endothelial cells exposed to shear stress
1Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Frontiers of Medical and Biological Engineering : the International Journal of the Japan Society of Medical Electronics and Biological Engineering
|January 1, 1993
Abstract:
Changes in patterns of microfilament distribution were analyzed using cultured porcine aortic endothelial cells under shear flow. Within the elongated endothelial cells after shear flow exposure (20 dyne/cm2, 0-24 h), F-actin filaments were mainly observed as stress fibers. After 24 h exposure, the fluorescence intensity of rhodamine-phalloidin-stained cells was enhanced, indicating the increase in F-actin content. It was suggested that not only F-actin distribution but also the content may be affected by shear stress at the early stage of exposure.