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Extracellular glycoprotein specific for Saccharomyces sensu stricto
S V Kononova1, A B Tsiomenko, W I Golubev
1Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino.
Abstract:
Using affinity-purified rabbit polyclonal antibodies against an extracellular mannoprotein (gp400) of Saccharomyces cerevisiae, the presence of immunohomologic proteins with similar electrophoretic mobility was shown in the culture medium of S. bayanus, S. paradoxus and S. pastorianus. Cross-reactive bands with different electrophoretic behaviour were observed for S. dairensis, S. exiguus, S. kluyveri, S. unisporus and also for the species moved from Saccharomyces to Arxiozyma, Kluyveromyces, Pachytichospora, Torulaspora and Zygosaccharomyces, in contrast to ascosporous yeasts of other genera in which these proteins were not found.