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Sequence specific interaction of DNA with water-soluble porphyrins
R Kuroda1, H Tanaka, S Watanabe
1Department of Chemistry, College of Arts and Sciences, University of Tokyo, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
Footprinting experiments and restriction enzyme inhibition work as well as affinity cleavage studies have been carried out to probe the sequence specific recognition of DNA by water-soluble free-base porphyrins and their metal complexes. Porphyrins lacking functional groups capable of forming hydrogen bonds exhibited high DNA sequence specificity. Induced circular dichroism (CD) spectroscopy was found useful in analyzing the complex DNA binding modes of these compounds.