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Misincorporation of ribonucleotides by DNA polymerase during in vitro DNA replication
1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
The fidelity of DNA replication with respect to misincorporation of ribonucleotides into DNA has been studied. Primed M13 DNA was replicated by E. coli DNA polymerase I in the presence of four dNTPs, a single rNTP and Mg2+ as a cofactor. All four rNTPs were incorporated into DNA in place of the corresponding dNTPs when rNTP/dNTP ratio was 1000:1. The frequency of rNTP misincorporation was C > G > A > U.