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Cloning and structural analysis of a human thymidylate synthase pseudogene splitted by several Alu sequences
K Takeishi1, Y Watanabe, K Isono
1Laboratory of Genetic Engineering, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
An unidentified genomic DNA fragment of 2.4kb that is weakly hybridizable with thymidylate synthase (TS) cDNA was cloned from a human genomic DNA library. Sequencing of the cloned DNA fragment and comparison of the sequence with that of the known human TS cDNA revealed that the DNA fragment contained a human TS processed pseudogene with unusual features. Based on the rate of nucleotide substitutions for neutral mutations in the 3'-untranslated regions between the gene and the pseudogene, it was estimated that the human TS pseudogene was formed about 16 million years ago.