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Controlling the conformation of large DNA molecule in aqueous solution
1Graduate School of Human Informatics, Nagoya University, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
We have studied the conformational change of T4DNA molecules in aqueous polyethylene glycol (PEG) solution by use of fluorescence microscopy. It was found that the DNA molecules exhibit abrupt transition from elongated coil into shrunken globule conformations depending on the concentration of PEG6000, a polymer with 186 monomer units. Interestingly, PEG20M, with 454 monomer units, shows two different types of transition; coil-globule transition around 0.1 M and globule-coil transition above 1 M. Such transitions are expected to be related to the regulation of gene expression and replication in living cells.