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Dialkylformamidine protected deoxyadenosine phosphoramidites in oligonucleotide synthesis
A Andrus1, P D Theisen, C McCollum
1Division of Perkin-Elmer Co., Foster City, CA 94404.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
A series of dialkylformamidine protected deoxyadenosine phosphoramidites were prepared for automated, solid-support DNA synthesis. The set of Abz, Gdmf, Cbz, T phosphoramidites gave high purity and high yield oligonucleotides, with rapid and complete deprotection at 65 degrees C in one hour. Different times and temperatures of exposure to concentrated ammonium hydroxide were examined to establish the optimum conditions for deprotection of oligonucleotides.