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Affinity gel electrophoresis of nucleic acids. The interaction between water soluble polymers having malachite green
Y Inami1, K Nakamura, H Ishiba
1Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
Copolymers prepared by the free radical copolymerization of acrylamide and vinyl malachite green were immobilized in an agarose gel matrix to produce a novel affinity gel for double-stranded DNAs. Relative electrophoretic mobilities of calf thymus DNA and poly(dA-dT)2 strongly depended on the amount of vinyl malachite green to show the strong interaction between DNAs and the copolymers. But any affinity interaction was not observed between poly(dG-dC)2 and the copolymers.