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Induced-fit association between DNA and esperamicin. Importance of structural flexibility in host DNA duplex
M Uesugi1, T Kusakabe, Y Sugiura
1Institute for Chemical Research, Kyoto University, Japan.
Nucleic Acids Symposium Series
|January 1, 1993
Abstract:
In this study, a series of synthetic oligonucleotide duplexes are tested as a substrate for esperamicin. The duplexes contain a typical binding sequence of esperamicin, 5'-GGA/TCC, but have different flexibilities in helix structure from each other. When cleavage activities of these oligonucleotides by esperamicin were estimated by using DNA sequencing method, a substantial increase of the cleavage at 3'-NAGG was observed with increasing the helix flexibility. This observation indicates that structural flexibility of host DNA duplex is important in an induced-fit association between esperamicin and DNA.