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Updated: Aug 3, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
[Comparative determination of cellular reactivity during infection with influenza virus A (H3N2)]
Abstract:
Among 28 strains of influenza A (H3N2) virus isolated from human patients 3 strains were found to be capable of replication in continuous monkey kidney cell cultures. When cells of different origins (PPO, chick embryo chorioallantio membrane, human nasal mucosa) were infected with the virus or when PPO were infected with allantoic or cultural virus strains, the pathological changes were of the same type. No specific inclusions were found in the cells. Adaptation of the virus to PPO cells resulted in inhibition of its hemagglutinating, neuraminidase and interferon-inducing activity and in decrease of thermostability, but the infectious and antgenic properties were retained unchanged.
Insights
Three of 28 influenza A (H3N2) virus strains replicated in monkey kidney cells. Adaptation to cell culture reduced viral activity but retained infectious and antigenic properties.
Area of Science:
- Virology
- Cell Biology
Background:
- Influenza A (H3N2) virus is a significant human pathogen.
- Understanding viral replication in cell cultures is crucial for influenza research.
Purpose of the Study:
- To investigate the replication of human influenza A (H3N2) virus in continuous monkey kidney cell cultures.
- To characterize the pathological changes and viral properties after adaptation to cell culture.
Main Methods:
- Isolation and culture of influenza A (H3N2) virus from human patients.
- Infection of various cell types (monkey kidney, chick embryo, human nasal mucosa) with influenza virus.
- Assessment of pathological changes, hemagglutinating activity, neuraminidase activity, interferon-inducing activity, thermostability, and antigenicity.
Main Results:
- Three out of 28 influenza A (H3N2) strains replicated in monkey kidney cell cultures.
- Pathological changes were similar across different cell types and virus strains.
- Adaptation to cell culture inhibited hemagglutinating, neuraminidase, and interferon-inducing activities, and decreased thermostability.
- Infectious and antigenic properties remained unchanged after cell culture adaptation.
Conclusions:
- Influenza A (H3N2) virus can adapt to replication in monkey kidney cell cultures.
- Cell culture adaptation alters specific viral activities while preserving infectivity and antigenicity.
- This adaptation provides a model for studying influenza virus behavior in vitro.
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