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Related Experiment Videos

Chronic vascular catheterization in the mouse

A Mokhtarian1, M J Meile, P C Even

  • 1Laboratoire de Neurobiologie des Régulations, CNRS UA 637, Collège de France, Paris.

Physiology & Behavior
|November 1, 1993
PubMed
Summary
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This study presents a novel surgical method for stress-free, periodic blood sampling and intravenous infusions in awake mice. The technique enables accurate monitoring of physiological parameters like blood glucose levels.

Area of Science:

  • Animal models in biomedical research
  • Surgical techniques for small animal research
  • Physiological monitoring in rodents

Background:

  • Accurate physiological monitoring in mice is crucial for research.
  • Existing methods for blood sampling can induce stress and affect results.
  • A need exists for minimally invasive techniques for long-term monitoring.

Purpose of the Study:

  • To describe a novel surgical method for stress-free, periodic blood sampling and intravenous infusions in awake mice.
  • To enable long-term monitoring of physiological parameters in behaving mice.
  • To facilitate studies such as glucose tolerance tests.

Main Methods:

  • Permanent implantation of a Silastic-rubber catheter in the right external jugular vein.

Related Experiment Videos

  • Subcutaneous tunneling of the catheter to the skull.
  • Fixation of the catheter using dental acrylic directly to the bone, avoiding screws.

    • Main Results:

    • The described technique allows for stress-free, periodic blood sampling in awake, behaving mice.
    • Intravenous infusions can also be administered using this method.
    • The method was successfully applied to perform glucose tolerance tests and monitor blood glucose levels over time.

    Conclusions:

    • This surgical approach provides a reliable and minimally invasive method for chronic catheterization in mice.
    • The technique reduces animal stress, leading to more accurate physiological data.
    • It is a valuable tool for various research applications requiring repeated blood sampling or infusions in mice.