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Production of parathyroid hormone-related protein by cultured human myeloma cells

A Suzuki1, T Takahashi, Y Okuno

  • 1Second Department of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto, Japan.

American Journal of Hematology
|January 1, 1994
PubMed

Insights

Parathyroid hormone-related protein (PTH-rP) is produced by human myeloma cells, potentially contributing to bone destruction and hypercalcemia in multiple myeloma. This study detected PTH-rP protein and mRNA in myeloma cell lines.

Area of Science:

  • Endocrinology
  • Oncology
  • Molecular Biology

Background:

  • Multiple myeloma is a cancer of plasma cells.
  • Bone destruction and hypercalcemia are common complications of multiple myeloma.
  • The role of parathyroid hormone-related protein (PTH-rP) in myeloma pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate the production of parathyroid hormone-related protein (PTH-rP) in cultured human myeloma cells.
  • To determine if PTH-rP expression correlates with myeloma-related complications.

Main Methods:

  • Radioimmunoassay was used to detect PTH-rP protein in myeloma cell extracts.
  • Reverse transcription polymerase chain reaction (RT-PCR) was employed to detect PTH-rP mRNA expression.

Main Results:

  • PTH-rP protein was detected in one out of six human myeloma cell lines (U266).
  • PTH-rP mRNA expression was demonstrated in all six examined myeloma cell lines.
  • These findings suggest PTH-rP is produced by myeloma cells.

Conclusions:

  • Parathyroid hormone-related protein (PTH-rP) is produced by human myeloma cells.
  • PTH-rP may be a contributing factor to the bone destruction observed in multiple myeloma.
  • PTH-rP may also play a role in the hypercalcemia associated with multiple myeloma.

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