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Updated: Oct 2, 2026

Quantitative Analysis of Chromatin Proteomes in Disease
Published on: December 28, 2012
Simultaneous determination of creatine compounds and adenine nucleotides in myocardial tissue by high-performance
T Teerlink1, M Hennekes, J Bussemaker
1Department of Clinical Chemistry, Free University Hospital, Amsterdam, The Netherlands.
Abstract:
A rapid high-performance liquid chromatography method was developed for the determination of creatine phosphate, creatine, adenine nucleotides, and related compounds in myocardial tissue. Analysis was performed by reversed-phase chromatography on a C18 column containing 3-microns particles, employing gradient elution and uv detection at 210 nm. Separation was achieved in less than 5 min. Total analysis time, including equilibration of the column after return of the gradient to starting conditions, was 8 min. The high reproducibility and short analysis time make this method suitable for the routine analysis of large series of samples.
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