Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acetone production by methylobacteria

A W Thomson, J G O'Neill, J F Wilkinson

    Archives of Microbiology
    |September 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Methylosinus trichosporium OB3B metabolizes ethane, producing acetone. This acetone originates from poly-beta-hydroxybutyric acid, involving key enzymes like acetoacetate decarboxylase.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characterization of eomesodermin and T-bet expression by allostimulated CD8<sup>+</sup> T cells of healthy volunteers and kidney transplant patients in relation to graft outcome.

    Clinical and experimental immunology·2018
    Same author

    A Tale of Two Pathways: Renewing the Promise of Anti-CD40L Blockade.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
    Same author

    Renal Allograft Survival in Nonhuman Primates Infused With Donor Antigen-Pulsed Autologous Regulatory Dendritic Cells.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
    Same author

    Impact of Human Mutant TGFβ1/Fc Protein on Memory and Regulatory T Cell Homeostasis Following Lymphodepletion in Nonhuman Primates.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
    Same author

    Regulatory T Cell Infusion Can Enhance Memory T Cell and Alloantibody Responses in Lymphodepleted Nonhuman Primate Heart Allograft Recipients.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same author

    In Vivo Mobilization and Functional Characterization of Nonhuman Primate Monocytic Myeloid-Derived Suppressor Cells.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same journal

    Disrupting virulence: in silico discovery of MexL inhibitors to block pyocyanin biosynthesis in Pseudomonas aeruginosa.

    Archives of microbiology·2026
    Same journal

    Genetic potential of Limosilactobacillus fermentum 5.1.2 produces metabolite compounds with antioxidant activity.

    Archives of microbiology·2026
    Same journal

    Stage-Specific carcinogenic mechanisms and intervention strategies for pks<sup>+</sup> Escherichia coli-associated colorectal cancer.

    Archives of microbiology·2026
    Same journal

    Improving high-degree-polymerization GOS synthesis of a marine cold-active β-galactosidase via semi-rational design.

    Archives of microbiology·2026
    Same journal

    Correction: Novel antimicrobial activity of protein produced by Streptomyces lividans TK24 against the phytopathogen Clavibacter michiganensis.

    Archives of microbiology·2026
    Same journal

    The AsnC family regulator VU14_03665 modulates antibiotic resistance and acid tolerance in Aeromonas hydrophila AH10.

    Archives of microbiology·2026
    See all related articles

    Area of Science:

    • Microbial metabolism
    • Biochemistry
    • Biotechnology

    Background:

    • Methylosinus trichosporium OB3B is a methane-oxidizing bacterium.
    • Ethane metabolism in microorganisms is not fully understood.
    • Acetone production can be a byproduct of microbial processes.

    Purpose of the Study:

    • To investigate the metabolic fate of ethane in Methylosinus trichosporium OB3B.
    • To identify the source and pathway of acetone accumulation during ethane metabolism.
    • To characterize the enzymatic machinery involved in acetone formation.

    Main Methods:

    • Cultivation of Methylosinus trichosporium OB3B under specific conditions.
    • Analysis of metabolic products using biochemical assays.
    • Enzyme activity assays for acetoacetate decarboxylase and 3-hydroxybutyrate dehydrogenase.

    Related Experiment Videos

    Main Results:

    • Acetone accumulation was observed during ethane and ethane oxidation product metabolism.
    • A decrease in poly-beta-hydroxybutyric acid correlated with acetone formation.
    • Presence of acetoacetate decarboxylase and 3-hydroxybutyrate dehydrogenase was confirmed.

    Conclusions:

    • A metabolic pathway for acetone production from poly-beta-hydroxybutyric acid is proposed.
    • This pathway involves 3-hydroxybutyrate and acetoacetate as intermediates.
    • Methylosinus trichosporium OB3B utilizes poly-beta-hydroxybutyric acid for acetone synthesis.