[Interaction of photoactive oligothymidylate derivatives with HeLa cell chromatin]

Photoactive derivatives of d(pT)16, bearing arylazide, nitroarylazide and perfluoroarylazide residues, were used for the complementary addressed modification of DNA and proteins in chromatin. As compared with alkylating derivatives, the photoactive compounds possess higher efficiency and specificity, and shorter incubation times which prevents nucleus from degradation. These reagents can therefore be used for identification of proteins located near to particular DNA regions in chromatin.