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Related Experiment Videos

Circulating and tissue endothelin immunoreactivity in hypercholesterolemic pigs

A Lerman1, M W Webster, J H Chesebro

  • 1Department of Internal Medicine, W.D.E., C.M.W., J.C.B.), Division of Cardiovascular Disease Mayo Clinic and Foundation, Rochester, Minn 55905.

Circulation
|December 1, 1993
PubMed
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High cholesterol in pigs elevates endothelin levels, a peptide that constricts blood vessels. This contributes to endothelial dysfunction and coronary vasoconstriction, indicating endothelin

Area of Science:

  • Cardiovascular Research
  • Endocrinology
  • Vascular Biology

Background:

  • Hypercholesterolemia is linked to impaired endothelium-dependent vasodilation, specifically a vasoconstrictive response to acetylcholine.
  • This dysfunction may stem from imbalances in endothelium-derived factors, including reduced relaxing factors and increased contracting factors.
  • Elevated circulating endothelin, a potent vasoconstrictor, is observed in conditions with abnormal vasodilation.

Purpose of the Study:

  • To investigate if experimental hypercholesterolemia increases plasma and tissue endothelin levels.
  • To determine if acetylcholine-induced coronary vasoconstriction in hypercholesterolemia is associated with elevated plasma endothelin.

Main Methods:

  • Determined plasma endothelin concentrations and molecular forms in pigs fed a high-cholesterol diet for 4 months.

Related Experiment Videos

  • Administered intracoronary acetylcholine to assess its effect on plasma endothelin and coronary artery response.
  • Utilized immunohistochemistry to evaluate endothelin presence in the coronary vascular wall.

    • Main Results:

    • Hypercholesterolemia significantly increased plasma endothelin concentrations and coronary artery tissue endothelin immunoreactivity.
    • Acetylcholine administration further elevated plasma endothelin in hypercholesterolemic pigs, coinciding with coronary vasoconstriction.
    • The primary circulating form of endothelin in hypercholesterolemia was identified as the biologically active endothelin-1.

    Conclusions:

    • Endothelin appears to play a role in the early stages of endothelial dysfunction associated with hypercholesterolemia.
    • Elevated endothelin serves as a potential marker for endothelial dysfunction in hypercholesterolemia.
    • Endothelin may contribute to the atherogenic process in the context of high cholesterol.